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Sample GSM379838 Query DataSets for GSM379838
Status Public on Jun 30, 2011
Title Patient2_339_GH_Day2_GH1_8PM (4 days)
Sample type RNA
 
Source name Milk Fat Globule
Organism Homo sapiens
Characteristics sample: Milk Fat Globule
Extracted molecule total RNA
Extraction protocol RNA was isolated from the milk fat globule using Trizol following the manufacturer's protocol
Label Biotin
Label protocol 440 ng total RNA was used for in vitro transcription, overnight for 14h using biotin-11-dUTP for labeling of the cRNA product using the Illumina®TotalPrep RNA amplification kit (Ambion, Inc., Austin, TX)
 
Hybridization protocol 750 ng of labeled cRNAs were hybridized to the Human Ref 8 V2 Beadchip arrays (Illumina Inc., San Diego, CA) at 55oC overnight (16h) following the Illumina Whole-Genome Gene Expression Protocol for BeadStation (Doc. # 111226048 Rev. B, Illumina Inc) and stained with 1 ug/ml streptavidin-Cy3 (Amersham Biosciences, Piscataway, NJ) for visualization
Scan protocol Probe arrays were scanned using the BeadArray reader (Factor 1)
Description Day2_GH1_8PM
Data processing Gene expression analysis was performed using the Sentrix BeadChip and BeadStation system from Illumina, Inc
 
Submission date Mar 12, 2009
Last update date Jun 30, 2011
Contact name Patricia Maningat
E-mail(s) [email protected]
Organization name Baylor College of Medicine
Department CNRC
Street address 1100 Bates St
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platform ID GPL6104
Series (1)
GSE15208 Gene Expression in the Human Mammary Epithelium during Lactation: the Milk Fat Globule Transcriptome through 4 Days

Data table header descriptions
ID_REF
VALUE cubic spline normalized signal intensity
Avg_NBEADS
BEAD_STDERR
Detection Pval

Data table
ID_REF VALUE Avg_NBEADS BEAD_STDERR Detection Pval
ILMN_1809034 181.7525 34 7.021366 0.001412429
ILMN_1660305 444.6314 36 14.24601 0
ILMN_1792173 190.5268 45 6.238465 0.001412429
ILMN_1762337 75.63308 28 4.881609 0.0960452
ILMN_1736007 57.60351 37 2.248657 0.8135593
ILMN_1787689 65.6135 53 2.080191 0.4039548
ILMN_1731507 52.90414 24 4.253098 0.9562147
ILMN_1814316 69.44034 39 2.745598 0.2288136
ILMN_1745607 65.58376 37 2.634857 0.4039548
ILMN_1757454 64.66801 41 2.546129 0.4392655
ILMN_1735045 73.98949 40 3.505749 0.1158192
ILMN_1680754 72.14696 39 2.685124 0.1454802
ILMN_1659452 62.05908 36 2.363688 0.5847458
ILMN_1767388 132.7972 43 5.737511 0.007062147
ILMN_1673870 60.56871 42 2.636307 0.6737288
ILMN_1675204 59.65018 30 2.816267 0.7231638
ILMN_1755321 121.2829 48 4.272331 0.008474576
ILMN_1698554 1524.338 33 42.75133 0
ILMN_1805938 59.76998 51 2.408221 0.7146893
ILMN_1676336 86.39733 37 3.519238 0.02118644

Total number of rows: 22184

Table truncated, full table size 912 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Processed data provided as supplementary file
Processed data are available on Series record
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