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Sample GSM379826 Query DataSets for GSM379826
Status Public on Jun 30, 2011
Title Patient3_339_GH_Day4_GH3_8AM (4 days)
Sample type RNA
 
Source name Milk Fat Globule
Organism Homo sapiens
Characteristics sample: Milk Fat Globule
Extracted molecule total RNA
Extraction protocol RNA was isolated from the milk fat globule using Trizol following the manufacturer's protocol
Label Biotin
Label protocol 440 ng total RNA was used for in vitro transcription, overnight for 14h using biotin-11-dUTP for labeling of the cRNA product using the Illumina®TotalPrep RNA amplification kit (Ambion, Inc., Austin, TX)
 
Hybridization protocol 750 ng of labeled cRNAs were hybridized to the Human Ref 8 V2 Beadchip arrays (Illumina Inc., San Diego, CA) at 55oC overnight (16h) following the Illumina Whole-Genome Gene Expression Protocol for BeadStation (Doc. # 111226048 Rev. B, Illumina Inc) and stained with 1 ug/ml streptavidin-Cy3 (Amersham Biosciences, Piscataway, NJ) for visualization
Scan protocol Probe arrays were scanned using the BeadArray reader (Factor 1)
Description Day4_GH3_8AM
Data processing Gene expression analysis was performed using the Sentrix BeadChip and BeadStation system from Illumina, Inc
 
Submission date Mar 12, 2009
Last update date Jun 30, 2011
Contact name Patricia Maningat
E-mail(s) [email protected]
Organization name Baylor College of Medicine
Department CNRC
Street address 1100 Bates St
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platform ID GPL6104
Series (1)
GSE15208 Gene Expression in the Human Mammary Epithelium during Lactation: the Milk Fat Globule Transcriptome through 4 Days

Data table header descriptions
ID_REF
VALUE cubic spline normalized signal intensity
Avg_NBEADS
BEAD_STDERR
Detection Pval

Data table
ID_REF VALUE Avg_NBEADS BEAD_STDERR Detection Pval
ILMN_1809034 154.6292 46 5.394334 0.001412429
ILMN_1660305 345.8514 29 13.36468 0
ILMN_1792173 206.8184 40 8.185683 0.001412429
ILMN_1762337 65.1191 49 1.92395 0.4336158
ILMN_1736007 61.35418 45 2.552762 0.6355932
ILMN_1787689 72.66819 59 2.58371 0.1313559
ILMN_1731507 52.43172 33 3.252532 0.9632769
ILMN_1814316 69.85982 41 3.066607 0.2175141
ILMN_1745607 66.90781 41 2.493625 0.3389831
ILMN_1757454 65.72681 43 2.030705 0.4081921
ILMN_1735045 76.7667 42 2.825997 0.06920904
ILMN_1680754 80.09841 59 2.521591 0.04378531
ILMN_1659452 60.86037 40 2.521945 0.6624294
ILMN_1767388 111.5006 35 3.906697 0.009887005
ILMN_1673870 63.39819 35 3.595626 0.529661
ILMN_1675204 59.54986 35 2.794302 0.7330508
ILMN_1755321 169.7744 55 5.190536 0.001412429
ILMN_1698554 920.813 39 30.6654 0
ILMN_1805938 61.04463 26 3.627288 0.6567796
ILMN_1676336 90.33327 46 2.766142 0.01129944

Total number of rows: 22184

Table truncated, full table size 906 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Processed data provided as supplementary file
Processed data are available on Series record
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