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Sample GSM379789 Query DataSets for GSM379789
Status Public on Jun 30, 2011
Title Patient5_339_GH_Day3_GH2_5PM (4 days)
Sample type RNA
 
Source name Milk Fat Globule
Organism Homo sapiens
Characteristics sample: Milk Fat Globule
Extracted molecule total RNA
Extraction protocol RNA was isolated from the milk fat globule using Trizol following the manufacturer's protocol
Label Biotin
Label protocol 440 ng total RNA was used for in vitro transcription, overnight for 14h using biotin-11-dUTP for labeling of the cRNA product using the Illumina®TotalPrep RNA amplification kit (Ambion, Inc., Austin, TX)
 
Hybridization protocol 750 ng of labeled cRNAs were hybridized to the Human Ref 8 V2 Beadchip arrays (Illumina Inc., San Diego, CA) at 55oC overnight (16h) following the Illumina Whole-Genome Gene Expression Protocol for BeadStation (Doc. # 111226048 Rev. B, Illumina Inc) and stained with 1 ug/ml streptavidin-Cy3 (Amersham Biosciences, Piscataway, NJ) for visualization
Scan protocol Probe arrays were scanned using the BeadArray reader (Factor 1)
Description Day3_GH2_5PM
Data processing Gene expression analysis was performed using the Sentrix BeadChip and BeadStation system from Illumina, Inc
 
Submission date Mar 12, 2009
Last update date Jun 30, 2011
Contact name Patricia Maningat
E-mail(s) [email protected]
Organization name Baylor College of Medicine
Department CNRC
Street address 1100 Bates St
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platform ID GPL6104
Series (1)
GSE15208 Gene Expression in the Human Mammary Epithelium during Lactation: the Milk Fat Globule Transcriptome through 4 Days

Data table header descriptions
ID_REF
VALUE cubic spline normalized signal intensity
Avg_NBEADS
BEAD_STDERR
Detection Pval

Data table
ID_REF VALUE Avg_NBEADS BEAD_STDERR Detection Pval
ILMN_1809034 153.4172 41 6.859761 0.001412429
ILMN_1660305 320.8651 33 15.72158 0
ILMN_1792173 198.5106 43 7.710474 0.001412429
ILMN_1762337 69.67342 41 2.475724 0.2189265
ILMN_1736007 65.93375 35 3.296386 0.3841808
ILMN_1787689 67.22568 38 2.841832 0.2980226
ILMN_1731507 54.1427 39 2.449815 0.9364407
ILMN_1814316 67.27259 58 2.088313 0.2980226
ILMN_1745607 61.55809 41 2.172446 0.6384181
ILMN_1757454 63.0442 49 2.833671 0.539548
ILMN_1735045 88.35072 47 3.939517 0.02118644
ILMN_1680754 85.19243 30 5.373571 0.0240113
ILMN_1659452 61.14355 39 3.109778 0.6539548
ILMN_1767388 108.841 46 4.988926 0.008474576
ILMN_1673870 65.93712 28 2.864705 0.3841808
ILMN_1675204 61.46777 35 2.991056 0.6412429
ILMN_1755321 157.9455 51 6.057505 0.001412429
ILMN_1698554 1001.038 42 35.53205 0
ILMN_1805938 60.42195 35 2.235503 0.6935028
ILMN_1676336 90.77686 34 2.87312 0.01836158

Total number of rows: 22184

Table truncated, full table size 905 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Processed data provided as supplementary file
Processed data are available on Series record
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