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Sample GSM379744 Query DataSets for GSM379744
Status Public on Jun 30, 2011
Title Patient4_339_GH_Day1_GH0_11AM (4 days)
Sample type RNA
 
Source name Milk Fat Globule
Organism Homo sapiens
Characteristics sample: Milk Fat Globule
Extracted molecule total RNA
Extraction protocol RNA was isolated from the milk fat globule using Trizol following the manufacturer's protocol
Label Biotin
Label protocol 440 ng total RNA was used for in vitro transcription, overnight for 14h using biotin-11-dUTP for labeling of the cRNA product using the Illumina®TotalPrep RNA amplification kit (Ambion, Inc., Austin, TX)
 
Hybridization protocol 750 ng of labeled cRNAs were hybridized to the Human Ref 8 V2 Beadchip arrays (Illumina Inc., San Diego, CA) at 55oC overnight (16h) following the Illumina Whole-Genome Gene Expression Protocol for BeadStation (Doc. # 111226048 Rev. B, Illumina Inc) and stained with 1 ug/ml streptavidin-Cy3 (Amersham Biosciences, Piscataway, NJ) for visualization
Scan protocol Probe arrays were scanned using the BeadArray reader (Factor 1)
Description Day1_GH0_2AM
Data processing Gene expression analysis was performed using the Sentrix BeadChip and BeadStation system from Illumina, Inc
 
Submission date Mar 12, 2009
Last update date Jun 30, 2011
Contact name Patricia Maningat
E-mail(s) [email protected]
Organization name Baylor College of Medicine
Department CNRC
Street address 1100 Bates St
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platform ID GPL6104
Series (1)
GSE15208 Gene Expression in the Human Mammary Epithelium during Lactation: the Milk Fat Globule Transcriptome through 4 Days

Data table header descriptions
ID_REF
VALUE cubic spline normalized signal intensity
Avg_NBEADS
BEAD_STDERR
Detection Pval

Data table
ID_REF VALUE Avg_NBEADS BEAD_STDERR Detection Pval
ILMN_1809034 117.0599 48 4.11277 0.007062147
ILMN_1660305 372.7725 43 9.094568 0
ILMN_1792173 180.2886 39 6.431191 0.001412429
ILMN_1762337 65.24557 54 3.108508 0.3940678
ILMN_1736007 68.70145 42 3.703172 0.25
ILMN_1787689 67.63088 35 2.557914 0.2824859
ILMN_1731507 56.65144 38 2.322076 0.8531073
ILMN_1814316 65.43898 51 2.631501 0.3855932
ILMN_1745607 87.90163 48 2.890838 0.0240113
ILMN_1757454 60.28308 32 3.108951 0.700565
ILMN_1735045 71.89232 49 2.7892 0.1370057
ILMN_1680754 78.40701 43 3.451489 0.06355932
ILMN_1659452 61.60235 50 2.319757 0.6440678
ILMN_1767388 124.4695 39 5.276484 0.007062147
ILMN_1673870 75.61246 36 3.449932 0.08898305
ILMN_1675204 63.86141 40 2.861209 0.4929379
ILMN_1755321 124.0721 43 4.561456 0.007062147
ILMN_1698554 1127.85 49 28.98874 0
ILMN_1805938 60.73588 41 2.645612 0.6793785
ILMN_1676336 108.0497 32 4.872477 0.008474576

Total number of rows: 22184

Table truncated, full table size 915 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Processed data provided as supplementary file
Processed data are available on Series record
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