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Sample GSM379740 Query DataSets for GSM379740
Status Public on Jun 30, 2011
Title Patient6_339_GH_Day4_GH3_2PM (4 days)
Sample type RNA
 
Source name Milk Fat Globule
Organism Homo sapiens
Characteristics sample: Milk Fat Globule
Extracted molecule total RNA
Extraction protocol RNA was isolated from the milk fat globule using Trizol following the manufacturer's protocol
Label Biotin
Label protocol 440 ng total RNA was used for in vitro transcription, overnight for 14h using biotin-11-dUTP for labeling of the cRNA product using the Illumina®TotalPrep RNA amplification kit (Ambion, Inc., Austin, TX)
 
Hybridization protocol 750 ng of labeled cRNAs were hybridized to the Human Ref 8 V2 Beadchip arrays (Illumina Inc., San Diego, CA) at 55oC overnight (16h) following the Illumina Whole-Genome Gene Expression Protocol for BeadStation (Doc. # 111226048 Rev. B, Illumina Inc) and stained with 1 ug/ml streptavidin-Cy3 (Amersham Biosciences, Piscataway, NJ) for visualization
Scan protocol Probe arrays were scanned using the BeadArray reader (Factor 1)
Description Day1_GH0_2PM
Data processing Gene expression analysis was performed using the Sentrix BeadChip and BeadStation system from Illumina, Inc
 
Submission date Mar 12, 2009
Last update date Jun 30, 2011
Contact name Patricia Maningat
E-mail(s) [email protected]
Organization name Baylor College of Medicine
Department CNRC
Street address 1100 Bates St
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platform ID GPL6104
Series (1)
GSE15208 Gene Expression in the Human Mammary Epithelium during Lactation: the Milk Fat Globule Transcriptome through 4 Days

Data table header descriptions
ID_REF
VALUE cubic spline normalized signal intensity
Avg_NBEADS
BEAD_STDERR
Detection Pval

Data table
ID_REF VALUE Avg_NBEADS BEAD_STDERR Detection Pval
ILMN_1809034 129.9381 52 4.352838 0.005649718
ILMN_1660305 299.0451 40 11.48637 0
ILMN_1792173 179.5161 37 5.888527 0.001412429
ILMN_1762337 65.3041 48 2.11339 0.4279661
ILMN_1736007 71.07599 42 2.625352 0.2033898
ILMN_1787689 68.84553 43 2.394833 0.2641243
ILMN_1731507 53.34698 48 2.152276 0.9590396
ILMN_1814316 67.24973 34 1.803179 0.3319209
ILMN_1745607 57.70523 64 1.45346 0.8333333
ILMN_1757454 64.14312 48 2.388228 0.4943503
ILMN_1735045 78.75185 38 2.729191 0.0720339
ILMN_1680754 77.23177 45 3.035289 0.09463277
ILMN_1659452 65.13924 38 2.59827 0.4392655
ILMN_1767388 105.6222 20 3.586862 0.01129944
ILMN_1673870 63.47623 33 3.618249 0.5466102
ILMN_1675204 63.15214 39 2.166636 0.5621469
ILMN_1755321 166.5278 52 4.677315 0.001412429
ILMN_1698554 1222.12 52 40.40083 0
ILMN_1805938 62.91503 44 2.566242 0.5861582
ILMN_1676336 80.14413 34 2.65196 0.05649718

Total number of rows: 22184

Table truncated, full table size 907 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Processed data provided as supplementary file
Processed data are available on Series record
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