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Sample GSM379725 Query DataSets for GSM379725
Status Public on Jun 30, 2011
Title Patient6_339_GH_Day2_GH1_2PM (4 days)
Sample type RNA
 
Source name Milk Fat Globule
Organism Homo sapiens
Characteristics sample: Milk Fat Globule
Extracted molecule total RNA
Extraction protocol RNA was isolated from the milk fat globule using Trizol following the manufacturer's protocol
Label Biotin
Label protocol 440 ng total RNA was used for in vitro transcription, overnight for 14h using biotin-11-dUTP for labeling of the cRNA product using the Illumina®TotalPrep RNA amplification kit (Ambion, Inc., Austin, TX)
 
Hybridization protocol 750 ng of labeled cRNAs were hybridized to the Human Ref 8 V2 Beadchip arrays (Illumina Inc., San Diego, CA) at 55oC overnight (16h) following the Illumina Whole-Genome Gene Expression Protocol for BeadStation (Doc. # 111226048 Rev. B, Illumina Inc) and stained with 1 ug/ml streptavidin-Cy3 (Amersham Biosciences, Piscataway, NJ) for visualization
Scan protocol Probe arrays were scanned using the BeadArray reader (Factor 1)
Description Day1_GH0_5PM
Data processing Gene expression analysis was performed using the Sentrix BeadChip and BeadStation system from Illumina, Inc
 
Submission date Mar 12, 2009
Last update date Jun 30, 2011
Contact name Patricia Maningat
E-mail(s) [email protected]
Organization name Baylor College of Medicine
Department CNRC
Street address 1100 Bates St
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platform ID GPL6104
Series (1)
GSE15208 Gene Expression in the Human Mammary Epithelium during Lactation: the Milk Fat Globule Transcriptome through 4 Days

Data table header descriptions
ID_REF
VALUE cubic spline normalized signal intensity
Avg_NBEADS
BEAD_STDERR
Detection Pval

Data table
ID_REF VALUE Avg_NBEADS BEAD_STDERR Detection Pval
ILMN_1809034 160.1941 40 7.643636 0.001412429
ILMN_1660305 305.0171 34 21.08668 0
ILMN_1792173 181.1055 53 7.407972 0.001412429
ILMN_1762337 73.05487 39 3.190548 0.1144068
ILMN_1736007 62.70705 41 2.643006 0.5466102
ILMN_1787689 72.10549 40 2.883608 0.1412429
ILMN_1731507 59.09363 32 2.494023 0.7768362
ILMN_1814316 62.70012 38 2.943679 0.5466102
ILMN_1745607 57.5703 29 3.180285 0.8502825
ILMN_1757454 63.14851 48 2.878943 0.5282486
ILMN_1735045 80.35627 44 4.2054 0.04519774
ILMN_1680754 73.88287 40 3.541991 0.09887005
ILMN_1659452 64.26018 41 3.019928 0.4646893
ILMN_1767388 103.7708 56 4.173935 0.009887005
ILMN_1673870 63.19775 36 2.99402 0.5268362
ILMN_1675204 62.80393 29 3.559878 0.539548
ILMN_1755321 158.9458 54 6.54802 0.001412429
ILMN_1698554 1358.41 56 63.47321 0
ILMN_1805938 59.22677 36 3.108715 0.769774
ILMN_1676336 76.57418 43 3.258243 0.06355932

Total number of rows: 22184

Table truncated, full table size 910 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Processed data provided as supplementary file
Processed data are available on Series record
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