|
| Status |
Public on Dec 15, 2009 |
| Title |
M22 60 min vs M22 T0 (2) - 25C to 37C heat shock |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
M22 grown at 25C
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: M22
|
| Treatment protocol |
Heat shock: cells were grown at 25C, then an aliquot of prewarmed medium was added to each culture to provide 37C upon mixing
|
| Growth protocol |
Unless otherwise noted, cells were grown at least 3 doublings in log phase in YPD medium at 25C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Hot-phenol RNA extraction as described in Gasch AP Methods in Enzymology 350: 393-414
|
| Label |
Oyster 550 cyanine dye
|
| Label protocol |
Indirect labeling using amino-allyl-dUTP and cyanine dyes from Flownamics (Madison, Wi) as described in Gasch AP Methods in Enzymology 350: 393-414
|
| |
|
| Channel 2 |
| Source name |
M22 - 60 min after 37C heat shock
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: M22
|
| Treatment protocol |
Heat shock: cells were grown at 25C, then an aliquot of prewarmed medium was added to each culture to provide 37C upon mixing
|
| Growth protocol |
Unless otherwise noted, cells were grown at least 3 doublings in log phase in YPD medium at 25C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Hot-phenol RNA extraction as described in Gasch AP Methods in Enzymology 350: 393-414
|
| Label |
Oyster cyanine 650 dye
|
| Label protocol |
Indirect labeling using amino-allyl-dUTP and cyanine dyes from Flownamics (Madison, Wi) as described in Gasch AP Methods in Enzymology 350: 393-414
|
| |
|
| |
| Hybridization protocol |
As indicated in Gasch AP Methods in Enzymology 350: 393-414
|
| Scan protocol |
Arrays were scanned on an Axon 4000B scanner
|
| Description |
25C to 37C heat shock timecourse in M22
yg7-30b
|
| Data processing |
Data were extracted using GenePix 6.0. Data were normalized by regional mean-centering as described by Lyne et al. 2003 BMC Genomics. Values in Sample tables have been adjusted such that a positive log2 value = induced by heat shock, and a negative log2 value = repressed by heat shock
|
| |
|
| Submission date |
Mar 06, 2009 |
| Last update date |
Dec 15, 2009 |
| Contact name |
Audrey P. Gasch |
| E-mail(s) |
[email protected]
|
| Phone |
(608)265-0859
|
| Fax |
(608)262-2976
|
| Organization name |
University of Wisconsin Madison
|
| Department |
Laboratory of Genetics
|
| Lab |
Gasch Lab
|
| Street address |
425-g Henry Mall
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53706 |
| Country |
USA |
| |
|
| Platform ID |
GPL5915 |
| Series (1) |
| GSE15147 |
Transient Genotype-by-Environment Interactions in the Yeast Genomic Expression Response to Heat Shock |
|
