|
| Status |
Public on Mar 06, 2009 |
| Title |
C-terminal truncated STAT5Δ750_178 |
| Sample type |
RNA |
| |
|
| Source name |
Mammary tumor
|
| Organism |
Mus musculus |
| Characteristics |
genotype: C-terminal truncated STAT5Δ750
tissue: mammary tumor
strain: FVB/N background
disease state: tumor
|
| Treatment protocol |
Tumors were excised and snap-frozen for RNA isolation.
|
| Growth protocol |
Mammary tumors were derived from transgenic mice carrying one of two Stat5 variants on a FVB/N background.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIZOL extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 15 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Hybridization was performed according to Affymetrix's protocol to Affymetrix GeneChip® Mouse Genome 430A 2.0 array.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from mammary tumor
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Mar 05, 2009 |
| Last update date |
Mar 18, 2009 |
| Contact name |
Tali Eilon |
| Organization name |
The Volcani Center
|
| Street address |
Rehovot
|
| City |
Rehovot |
| ZIP/Postal code |
17000 |
| Country |
Israel |
| |
|
| Platform ID |
GPL8321 |
| Series (1) |
| GSE15119 |
Mammary tumors in transgenic mice expressing constitutively active and C-terminally truncated variants of STAT5 |
|
