|
| Status |
Public on Nov 26, 2019 |
| Title |
PVWM-17-007 |
| Sample type |
SRA |
| |
|
| Source name |
PVWM
|
| Organism |
Ovis aries |
| Characteristics |
gestational age (days): 127
Sex: M
mechanical ventilation: Ventilated
treament 1: Intratracheal Poractant alpha
dose surfactant: 2.5mL/kg
treament 2: None
dose budesonide: N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted with Trizol per manufacturer's guidelines.
Afer polyA selection, paired-end RNA-sequencing with 75bp was done with a depth of 30M reads.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
PVWM-17-007
processed data file: counts_pvwm.csv
|
| Data processing |
Alignment to the Oar4.0 done with Bowtie2
Read counts was done using FeatureCounts
Differential expression analysis was performed with EdgeR
Genome_build: OAR4.0
Supplementary_files_format_and_content: readcounts
|
| |
|
| Submission date |
May 14, 2019 |
| Last update date |
Nov 26, 2019 |
| Contact name |
Augusto F Schmidt |
| E-mail(s) |
[email protected]
|
| Phone |
3052430156
|
| Organization name |
University of Miami Miller School of Medicine
|
| Street address |
1611 NW 12th ave
|
| City |
Miami |
| State/province |
FL |
| ZIP/Postal code |
33136 |
| Country |
USA |
| |
|
| Platform ID |
GPL15670 |
| Series (1) |
| GSE131195 |
Surfactant plus budesonide decreases lung and systemic responses to injurious ventilation in preterm sheep |
|
| Relations |
| BioSample |
SAMN11640934 |
| SRA |
SRX5830497 |
