|
| Status |
Public on Nov 23, 2009 |
| Title |
mSMC-TNF-24h-0 |
| Sample type |
RNA |
| |
|
| Source name |
Aortic Smooth Muscle Cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J, Gender: male, Age: 10-12 weeks, Tissue: aorta
|
| Treatment protocol |
Cells were feed 24h prior to stimulation, stimuli were added in PBS, Cells were stimulated with 1ng/ml TNFalpha
|
| Growth protocol |
Maintained in DMEM/F12, 10% FCS, feed every 48h
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNEasy Lipid Tissue Mini Kit, Standard protocol
|
| Label |
Biotin/SAPE with Anti Streptavidin signal amplification
|
| Label protocol |
ENZO T7 High Yield Transcript Labelling Kit, 16h IVT
|
| |
|
| Hybridization protocol |
60rpm, 18h, 45°C in Affymetrix Hybridization Buffer
|
| Scan protocol |
Affymetrix Scanner 3000, single scan
|
| Description |
Staining with Fluidic Station 450 Protocol: EukGe-WS2v4
|
| Data processing |
GCOS 1.4, scaled to 500
|
| |
|
| Submission date |
Mar 02, 2009 |
| Last update date |
Nov 23, 2009 |
| Contact name |
Andreas Johann Richard Habenicht |
| E-mail(s) |
[email protected]
|
| Organization name |
Ludwig Maximilians Universität München
|
| Department |
Medicine
|
| Lab |
Institute for Cardiovascular Disease Prevention
|
| Street address |
Pettenkoferstraße 9a
|
| City |
Munich |
| State/province |
Bavaria |
| ZIP/Postal code |
80336 |
| Country |
Germany |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE15062 |
Mouse aorta smooth muscle cells differentiate into lymphoid tissue organizers upon combined TNFR1/LTBR NF-kB signaling |
|
