|
| Status |
Public on Jun 08, 2020 |
| Title |
Skeletal Muscle Exe1 |
| Sample type |
SRA |
| |
|
| Source name |
Skeletal Muscle
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Muscle satellite cells (SCs)
cell states: Trained
treatment: Exercise
|
| Treatment protocol |
The mice in the exercise groups were subjected to 4 weeks of running exercise (4 days/week, 30 min/day at 12 m/min)
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
About 800 SCs were isolated by FACS and the mRNA library constructed using QIAseq FX Single Cell RNA Library Kit (QIAGEN) according to the manufacturer’s instruction
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Illumina Casava1.7 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm10 whole genome using bowtie v0.12.9.
Reads Per Kilobase of exon per Megabase of library size (RPKM) was performed with Cufflinks v2.1.1, aligned reads were counted with HTSeq.
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files include RPKM values for each Sample ...
|
| |
|
| Submission date |
Mar 21, 2019 |
| Last update date |
Jun 08, 2020 |
| Contact name |
Zhe Chen |
| E-mail(s) |
[email protected]
|
| Organization name |
Chongqing Medical University
|
| Street address |
1 Yixueyuan Road, Yuzhong District
|
| City |
Chongqing |
| ZIP/Postal code |
400016 |
| Country |
China |
| |
|
| Platform ID |
GPL13112 |
| Series (1) |
| GSE128651 |
Next Generation Sequencing Facilitates Quantitative Analysis of SCs in mice exposure to exercise |
|
| Relations |
| BioSample |
SAMN11189834 |
| SRA |
SRX5550251 |
