|
| Status |
Public on Dec 22, 2009 |
| Title |
HCT-8_indole_4h_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
HCT-8 cell monolayer treated with indole for 4 hr
|
| Organism |
Homo sapiens |
| Characteristics |
Human colon-cancer cell line HCT-8
|
| Extracted molecule |
total RNA |
| Extraction protocol |
HCT-8 cells were grown in monolayers till 80% confluence. Indole was added to cells and they were incubated for further 4h or 24 h. RNA was extracted using Qiagen RNeasy Kit.
|
| Label |
biotin, Alexa-647 Streptavidin
|
| Label protocol |
CodeLink Gene Expression System: Manual Labelled cRNA Target Preparation according to manufacturer's instructions http://www.appliedmicroarrays.com/pdf/iexpress2006_web.pdf
|
| |
|
| Hybridization protocol |
CodeLink Gene Expression System: Single-Assay Bioarray Hybridization and Detection according to manufacturer's instructions http://www.appliedmicroarrays.com/pdf/codelink_bioarray_processing_protocol.pdf
|
| Scan protocol |
CodeLink Gene Expression System: Single-Assay Bioarray Hybridization and Detection according to manufacturer's instructions http://www.appliedmicroarrays.com/application_notes/63005079.pdf
|
| Description |
Cells were grown in RPMI 1640 medium
|
| Data processing |
Median normalized values derived from CodeLink Expression Analysis Version 4.1.0.29054
|
| |
|
| Submission date |
Jan 12, 2009 |
| Last update date |
Dec 22, 2009 |
| Contact name |
Tarun Bansal |
| E-mail(s) |
[email protected]
|
| Phone |
979-661-4318
|
| Organization name |
Texas A & M University
|
| Department |
Chemical Engineering
|
| Lab |
Prof Thomas Wood Lab
|
| Street address |
3122 TAMU
|
| City |
College Station |
| State/province |
TX |
| ZIP/Postal code |
77843 |
| Country |
USA |
| |
|
| Platform ID |
GPL8060 |
| Series (1) |
| GSE14379 |
Human epithelial cell response to bacterial signal indole |
|
