|
| Status |
Public on Jun 27, 2019 |
| Title |
Control sample 26 |
| Sample type |
genomic |
| |
|
| Source name |
Blood
|
| Organism |
Homo sapiens |
| Characteristics |
Sex: M
age (years): 1
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from blood samples using DNEasy (Qiagen) chemistry and columns. No modification was made to the manufacturer's specifications. Prior to extraction, red cells were lysed by exposure of the sample to 2x Lysis Buffer for 5 minutes in ice. The remaining cells were pelletted by centrifugation at 2300 RPM at 4 degrees celsius.
|
| Label |
Cy3 and Cy5
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
| Scan protocol |
Using an Illumina HiScan
|
| Description |
Genomic DNA extracted from blood had DNA methylation measured using the EPIC array (Illumina)
|
| Data processing |
Using an Illumina HiScan
|
| |
|
| Submission date |
Jan 18, 2019 |
| Last update date |
Jul 26, 2019 |
| Contact name |
Eric J Chater-Diehl |
| E-mail(s) |
[email protected]
|
| Organization name |
SickKids Research Institute
|
| Lab |
Rosanna Weksberg
|
| Street address |
686 Bay St.
|
| City |
Toronto |
| State/province |
ON |
| ZIP/Postal code |
M5G 0A4 |
| Country |
Canada |
| |
|
| Platform ID |
GPL21145 |
| Series (1) |
| GSE125367 |
Differental DNA methylation in Nicolaides-Baraitser syndrome |
|
