|
| Status |
Public on Mar 16, 2009 |
| Title |
PDGF-BB stimulation for 1h_replicate2 |
| Sample type |
RNA |
| |
|
| Source name |
AG01518 forskin human fibroblasts
|
| Organism |
Homo sapiens |
| Characteristics |
Normal Fibroblasts
|
| Treatment protocol |
Cells were stimulated for 24h or 1h by PDGF-BB (25 ng/ml) or b-FGF (10 ng/ml + heparine 50 microg/ml)
|
| Growth protocol |
Human foreskin fibroblasts (AG01518) were cultured (1.5E6 cells/10 cm dish) in MEM medium with 10% fetal calf serum and glutamine for 24h, then washed and incubated for 47h in serum-free medium. Cells were stimulated for 1h by PDGF-BB (25ng/ml) or b-FGF (10ng/ml, + heparine 50 microg/ml). Alternatively, cells were incubated for 24h in starvation medium and treated for 24h with growth factors, or left untreated for 48h in serum-free medium (control treatment).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini Kit (Qiagen)
|
| Label |
Biotin
|
| Label protocol |
Standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol
|
| Scan protocol |
Standard Affymetrix protocol
|
| Description |
1h PDGF-BB stimulation
|
| Data processing |
MAS5 (GCOS) : the scaling factor using all probe sets was set to 100 and the normalization factor was set to 1
|
| |
|
| Submission date |
Dec 31, 2008 |
| Last update date |
Mar 16, 2009 |
| Contact name |
Jean-Baptiste Demoulin |
| Organization name |
Université catholique de Louvain
|
| Department |
de Duve Institute
|
| Lab |
UCL - MEXP
|
| Street address |
av Hippocrate 75, B1.74.05
|
| City |
Brussels |
| ZIP/Postal code |
B-1200 |
| Country |
Belgium |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE14256 |
Human fibroblast stimulation with PDGF-BB or b-FGF |
|
