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| Status |
Public on Jun 24, 2019 |
| Title |
Cyclo_20min |
| Sample type |
SRA |
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| Source name |
whole organism
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| Organism |
Saccharomyces cerevisiae W303 |
| Characteristics |
strain: W303
genotype: W303 background;leu2-3,112 his3-11,15 ura3-1 ade2-1 trp1-1 can1-100 MATa
treatment: Cycloheximide
antibody: Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) (ab5131)
time: 20 minutes after treatment
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| Growth protocol |
cells were grown at 30 degrees in YPAD to mid-log phase before heat shock
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| Extracted molecule |
genomic DNA |
| Extraction protocol |
TruSeq ChIP library preparation kit (Illumina)
ChIP-seq was performed as described previously (Ribaud et al., 2012) (PMID: 21952045).
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| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 2500 |
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| Data processing |
For ChIP-Seq raw reads were aligned to the sacCer3
For ChIP-Seq, data were filtered to retain only the mapped reads with at most 5 hits in the reference.
For ChIP-Seq, reads that mapped to both sacCer3
For ChIP-Seq, the reads were shifted by 100 bp and extended by 50 bp
Genome_build: sacCer3
Supplementary_files_format_and_content: wig and bigwig files were obtained using HTSStation (http://htsstation.epfl.ch)
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| Submission date |
Jan 17, 2019 |
| Last update date |
Jun 26, 2019 |
| Contact name |
Benjamin Albert |
| E-mail(s) |
[email protected]
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| Organization name |
University of Genevea
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| Department |
Bimol
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| Lab |
David Shore
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| Street address |
30, quai Ernest-Ansermet
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| City |
GENEVA |
| ZIP/Postal code |
CH-1211 |
| Country |
Switzerland |
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| Platform ID |
GPL25517 |
| Series (1) |
| GSE125226 |
A ribosome assembly stress response regulates transcription to maintain proteome homeostasis |
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| Relations |
| BioSample |
SAMN10762469 |
| SRA |
SRX5257259 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM3566682_Cyclo_20min_merged.bw |
40.4 Mb |
(ftp)(http) |
BW |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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