|
| Status |
Public on Sep 03, 2019 |
| Title |
DGM-00661_lg (array) |
| Sample type |
RNA |
| |
|
| Source name |
large airway epithelium brushing
|
| Organism |
Homo sapiens |
| Characteristics |
smoking status: nonsmoker
age: 35
gender: M
ethnicity: Afr
tissue: large airway epithelium brushing
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction and RNAeasy clean-up of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1-2 microg total RNA (Expression Analysis Technical Manual, 701022 Rev.2, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G.
|
| Data processing |
The data were analyzed with Partek Genomics Suite software, version 6.6 using default analysis settings and robust multi-array (RMA) normalization method.
|
| |
|
| Submission date |
Dec 21, 2018 |
| Last update date |
Sep 03, 2019 |
| Contact name |
Yael Strulovici-Barel |
| E-mail(s) |
[email protected]
|
| Organization name |
Weill Cornell Medical College
|
| Department |
Department of Genetic Medicine
|
| Lab |
Crystal
|
| Street address |
1300 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE124253 |
Characterization of an Immortalized Human Small Airway Basal Stem/Progenitor Cell Line with Airway Region-specific Differentiation Capacity [array] |
| GSE124265 |
Characterization of an Immortalized Human Small Airway Basal Stem/Progenitor Cell Line with Airway Region-specific Differentiation Capacity |
|
