|
| Status |
Public on Dec 02, 2019 |
| Title |
Drosophila Melanogaster_zt23_18C |
| Sample type |
SRA |
| |
|
| Source name |
fly heads
|
| Organism |
Drosophila melanogaster |
| Characteristics |
strain: Canton-S
circadian timepoints: zt23
temperature: 18C
tissue: fly heads
|
| Treatment protocol |
RNA extraction was done using TRI Reagent [SIGMA] following manufacturer´s protocol.
|
| Growth protocol |
Flies were grown at different temperaturesÂ
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
The precipitated RNAwas taken for library preparation.
Libraries were constructed using the protocol for ligation base libraries (Engreitz et al., 2013)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
transcript_avg(per_bp)_18Czt23
|
| Data processing |
Ribosomal RNA was taken out using Bowtie2 aligner against the ribosomal RNA
Reads were aligned to dm3 genome using TopHat
The aligned reads were analyzed with cufflinks
Genome_build: dm3
Supplementary_files_format_and_content: txt file with isoforms quantification
|
| |
|
| Submission date |
Dec 19, 2018 |
| Last update date |
Dec 02, 2019 |
| Contact name |
Sebastian Kadener |
| E-mail(s) |
[email protected]
|
| Organization name |
Brandeis University
|
| Street address |
415 South Street
|
| City |
Waltham |
| State/province |
MA |
| ZIP/Postal code |
9190401 |
| Country |
USA |
| |
|
| Platform ID |
GPL19132 |
| Series (2) |
| GSE124135 |
RNA seq from fly heads at different temperatures and circadian timepoints [independent replicate 1] |
| GSE124136 |
Differences in circadian rhythms in fly at different temperatures |
|
| Relations |
| BioSample |
SAMN10616365 |
| SRA |
SRX5165613 |
