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Sample GSM3510289 Query DataSets for GSM3510289
Status Public on Jun 10, 2019
Title presurgical patient 015 [granulocytes]
Sample type RNA
 
Source name peripheral granulocytes from presurgical patient
Organism Homo sapiens
Characteristics diagnosis: presurgical
cell type: peripheral granulocytes
Treatment protocol Granulocytes were prepared from 2 mL blood by positive selection (StraightFrom™ Whole Blood CD15 MicroBeads, Miltenyi Biotec).
Growth protocol Blood was collected in S-Monovette® K3E tubes (Sarstedt, Nümbrecht, Germany) by venipuncture with presurgical patients and from a central venous catheter with ICU patients.
Extracted molecule total RNA
Extraction protocol Total RNA was purified from isolated granulocytes stored in RNAlater® (Ambion) using the miRVana miRNA isolation kit (Applied Biosystems).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
Scan protocol Affymetrix GeneArray Scanner3000.
Description S_B_015
Gene expression data from peripheral granulocytes of presurgical patient.
Data processing The data were analyzed with a commercial software called JMP Genomics, version 6, from SAS. Gene expression profiling was performed using arrays of human Hugene-2_0-type from Affymetrix. A Custom CDF Version 20 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization, RMA background correction and Medianpolish Probeset Summary.
 
Submission date Dec 12, 2018
Last update date Jun 10, 2019
Contact name Carsten Sticht
Organization name University Heidelberg
Department ZMF
Street address Theodor-Kutzer-Ufer
City Mannheim
ZIP/Postal code 68169
Country Germany
 
Platform ID GPL21970
Series (2)
GSE123729 AKIRIN1 is a suitable reference gene in natural killer cells and granulocytes from patients with SIRS and septic shock [granulocytes]
GSE123731 AKIRIN1 is a suitable reference gene in natural killer cells and granulocytes from patients with SIRS and septic shock

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
100009613_at 3.220703125
100009676_at 4.590820313
10000_at 2.860351563
10001_at 5.75
10002_at 3.283691406
100033411_at 2.956054688
100033413_at 2.696289063
100033422_at 2.585449219
100033423_at 2.842773438
100033424_at 2.463378906
100033425_at 2.34375
100033426_at 7.172851563
100033427_at 9.345703125
100033428_at 2.240722656
100033430_at 2.349609375
100033431_at 4.405273438
100033432_at 2.260253906
100033434_at 2.331054688
100033435_at 8.310546875
100033436_at 2.593261719

Total number of rows: 24733

Table truncated, full table size 511 Kbytes.




Supplementary file Size Download File type/resource
GSM3510289_Lindner_230516_S-B-015_HuGene-2_0-st_.CEL.gz 7.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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