|
| Status |
Public on Dec 05, 2018 |
| Title |
sog1_bleomycin_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
sog1_roots_bleomycin
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype background: Col-0
genotype/variation: sog1-101
age: Five-day-old plant
treatment: 0.6 µg/ml bleomycin for 10 h
tissue: roots
|
| Treatment protocol |
Five-day-old WT, anac044-1 anac085-1, and sog1-101 plants were transferred to MS medium with or without 0.6 µg/ml bleomycin for 10 h.
|
| Growth protocol |
Arabidopsis plants were grown in Murashige and Skoog (MS) medium under continuous light at 23 ˚C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from two biological replicates with Plant Total RNA Mini Kit (FAVORGEN) according to the manufacturer’s instructions.
|
| Label |
Cy3
|
| Label protocol |
200 ng of total RNA was used to prepare for Cy3-labeled cRNA, using Low Input Quick Amp Labeling Kit (Agilent).
|
| |
|
| Hybridization protocol |
600 ng of cRNAs were hybridized to an Arabidopsis Agilent custom microarrays at 65 ˚C. for 17 h, using Gene Expression Hybridization kit. After hybridization, the microarrays were washed with Gene Expression Wash Buffer 1 (Agilent) and Gene Expression Wash Buffer (Agilent), then dried immediately by brief centrifugation.
|
| Scan protocol |
The microarrays were scanned by Agilent DNA Microarray Scanner (G2539A ver.C).
|
| Description |
Gene expression after bleomycin treatment
US45103077_253459210074_S01_GE1_107_Sep09_2_4
|
| Data processing |
When Agilent feature extraction software outputted raw data, the software omitted rows associated with spots where DNA proves were not spotted.
|
| |
|
| Submission date |
Dec 04, 2018 |
| Last update date |
Dec 05, 2018 |
| Contact name |
Naoki Takahashi |
| Organization name |
Nara Institute of Science and Technology
|
| Street address |
Takayama 8916-5
|
| City |
Ikoma |
| State/province |
Nara |
| ZIP/Postal code |
630-0192 |
| Country |
Japan |
| |
|
| Platform ID |
GPL22706 |
| Series (1) |
| GSE123315 |
Transcriptomic analysis of Arabidopsis anac044anac085 and sog1 mutnat under DNA damage condition. |
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