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Sample GSM347152 Query DataSets for GSM347152
Status Public on Dec 02, 2009
Title CRT knockout mouse embryonic stem cell, biological rep1
Sample type RNA
 
Source name Calreticulin knockout of mouse embryonic stem cell line
Organism Mus musculus
Characteristics Strain: D3, mouse embryonic stem cell
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix 430 2.0 Mouse Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000
Description Gene expression data from murine embryonic stem cells with calreticulin deficiency
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Dec 03, 2008
Last update date Dec 03, 2008
Contact name Randolph Faustino
E-mail(s) [email protected]
Organization name Mayo Clinic
Street address 200 First Street SW
City Rochester
State/province MN
ZIP/Postal code 55905
Country USA
 
Platform ID GPL1261
Series (1)
GSE13805 Expression data from wild type and calreticulin deficient murine embryonic stem cells

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 871.6 P 0.00039
AFFX-BioB-M_at 1616.9 P 4.4e-05
AFFX-BioB-3_at 957.5 P 4.4e-05
AFFX-BioC-5_at 2060.3 P 6e-05
AFFX-BioC-3_at 1790.3 P 4.4e-05
AFFX-BioDn-5_at 2881.7 P 4.4e-05
AFFX-BioDn-3_at 9217.8 P 8.1e-05
AFFX-CreX-5_at 19152 P 5.2e-05
AFFX-CreX-3_at 23301.8 P 4.4e-05
AFFX-DapX-5_at 8098.9 P 4.4e-05
AFFX-DapX-M_at 15589.6 P 5.2e-05
AFFX-DapX-3_at 16927.9 P 4.4e-05
AFFX-LysX-5_at 2195.8 P 4.4e-05
AFFX-LysX-M_at 3135.5 P 4.4e-05
AFFX-LysX-3_at 6748.9 P 4.4e-05
AFFX-PheX-5_at 3.3 A 0.834139
AFFX-PheX-M_at 2.3 A 0.904333
AFFX-PheX-3_at 12.7 A 0.686277
AFFX-ThrX-5_at 6.8 A 0.724854
AFFX-ThrX-M_at 2.7 A 0.645559

Total number of rows: 45101

Table truncated, full table size 1214 Kbytes.




Supplementary file Size Download File type/resource
GSM347152.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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