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Status |
Public on Dec 02, 2009 |
Title |
CRT knockout mouse embryonic stem cell, biological rep1 |
Sample type |
RNA |
|
|
Source name |
Calreticulin knockout of mouse embryonic stem cell line
|
Organism |
Mus musculus |
Characteristics |
Strain: D3, mouse embryonic stem cell
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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|
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Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix 430 2.0 Mouse Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000
|
Description |
Gene expression data from murine embryonic stem cells with calreticulin deficiency
|
Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
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|
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Submission date |
Dec 03, 2008 |
Last update date |
Dec 03, 2008 |
Contact name |
Randolph Faustino |
E-mail(s) |
[email protected]
|
Organization name |
Mayo Clinic
|
Street address |
200 First Street SW
|
City |
Rochester |
State/province |
MN |
ZIP/Postal code |
55905 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE13805 |
Expression data from wild type and calreticulin deficient murine embryonic stem cells |
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