|
| Status |
Public on Sep 09, 2019 |
| Title |
E16.5_1 |
| Sample type |
RNA |
| |
|
| Source name |
C57Bl6J embryo
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: C57Bl6J
genotype/variation: WT
developmental stage: E16.5
tissue: Brown adipose tissue (BAT)
|
| Treatment protocol |
Mice and embryos did not receive any treatment.
|
| Growth protocol |
Mice were housed in a temperature-controlled room (22–24°C) with a relative humidity of 36%–80%, and 12-hour light/12-hour dark cycles. Animal studies were performed in compliance with European Community specifications regarding the use of laboratory animals and approved by the Nord-Pas de Calais Ethical Committee for animal use. Embryos were dated taking Embryonic day (E) 0.5 as the day after the vaginal plug.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using RNeasy Microkit (Qiagen). cDNA was obtained with a reverse transcription Kit (High-capacity cDNA reverse transcription Kit, Life Technologies). Total RNA was checked for quantity and quality using the Agilent 2100 Bioanalyzer (Agilent Biotechnologies).
|
| Label |
Biotin
|
| Label protocol |
RNA were processed using Affymetrix GeneChip® WT PLUS Reagent Kit.
|
| |
|
| Hybridization protocol |
Affymetrix GeneChip Hybridization Oven 640 for 16h at 45°C and 60 rpm on MoGene-2_1-st Chip. GeneChips were washed and stained in the Affymetrix Fluidics.
|
| Scan protocol |
Affymetrix GeneChip Scanner 7G and Affymetrix GeneChip Command Console software (AGCC).
|
| Description |
Transcriptomic MPH
|
| Data processing |
Raw transcriptomic data from Affymetrix microarrays were normalized using the Partek Genomics Suite (v7.0) using background correction by Robust Multi-array Average (RMA), quantile normalization and summarization via median-polish. RMA values from different probes matching to the same gene were averaged.
|
| |
|
| Submission date |
Nov 09, 2018 |
| Last update date |
Sep 09, 2019 |
| Contact name |
Jérôme Eeckhoute |
| E-mail(s) |
[email protected]
|
| Organization name |
CNRS
|
| Lab |
INSERM UMR 1011, Université Lille-Nord de France
|
| Street address |
Bâtiment J&K, Faculté de Médecine de Lille-Pôle Recherche, Boulevard du Professeur Leclerc
|
| City |
Lille |
| ZIP/Postal code |
59045 |
| Country |
France |
| |
|
| Platform ID |
GPL25793 |
| Series (1) |
| GSE122395 |
Transcriptomic analysis of embryonic brown adipose tissue (BAT) |
|
