|
| Status |
Public on Nov 17, 2008 |
| Title |
ago1-25 biological replicate 2 |
| Sample type |
SRA |
| |
|
| Source name |
Inflorescence tissue: flower stages 1-12
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
Col-0 ecotype, ago1-25 homozygous plants
|
| Growth protocol |
Plants were maintained on soil in a standard greenhouse with 16 hr light/8 hr dark supplemental light cycle.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from Inflorescence tissue (flower stages 1-12) using Trizol and chloroform. Following isolation the RNA was purified by phenol-chloroform extraction. Small RNA were fractionated on denaturing 17% polyacrylamide gels. Amplicons for sequencing were prepared by 5’ and 3’ adaptor ligations followed by RT-PCR. Sequencing-by-synthesis was done using an Illumina 1G Genome Analyzer.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina Genome Analyzer |
| |
|
| Description |
ago1-25 biological replicate 2
|
| Data processing |
Small RNA sequences were parsed from Illumina sequencing data files through identification of adaptor boundaries. Only sequences that mapped to the Arabidopsis thaliana Col-0 genome were included in the analysis.
|
| |
|
| Submission date |
Nov 14, 2008 |
| Last update date |
May 15, 2019 |
| Contact name |
James C Carrington |
| E-mail(s) |
[email protected]
|
| Phone |
314-587-1202
|
| Organization name |
Donald Danforth Plant Science Center
|
| Lab |
James C. Carrington
|
| Street address |
975 North Warson Road
|
| City |
Saint Louis |
| State/province |
MO |
| ZIP/Postal code |
63132 |
| Country |
USA |
| |
|
| Platform ID |
GPL9062 |
| Series (1) |
| GSE13605 |
AGO1-dependent Small RNA in Arabidopsis Identified by High-Throughput Sequencing |
|
| Relations |
| SRA |
SRX003108 |
| BioSample |
SAMN02195596 |
