|
| Status |
Public on Oct 22, 2009 |
| Title |
Control |
| Sample type |
RNA |
| |
|
| Source name |
human dermal skin fibroblasts
|
| Organism |
Homo sapiens |
| Characteristics |
sham treated human dermal skin fibroblasts.
Human neonatal skin fibroblasts (FB) were obtained from Lifeline Cell Technology; Walkersville, MD and grown in Dulbecco's modified Eagle's medium, Gibco (Gaithersburg, MD) supplemented with 10% fetal calf serum (FCS) and penicillin/ streptomycin (1000U per ml, Gibco) to sub-confluence. Fourth passage FB were used at a confluence of 60-70% in 12-well tissue culture plates.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol followed by RNeasy Mini kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
According to standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
According to standard Affymetrix protocol.
|
| Scan protocol |
According to standard Affymetrix protocol.
|
| Description |
No additional information.
|
| Data processing |
MAS 5.0
|
| |
|
| Submission date |
Nov 14, 2008 |
| Last update date |
Oct 22, 2009 |
| Contact name |
Martin Bilban |
| E-mail(s) |
[email protected]
|
| Phone |
++43 (0)1 40400 6441
|
| Organization name |
Medical University of Vienna
|
| Department |
|
| Street address |
|
| City |
Vienna |
| ZIP/Postal code |
1090 |
| Country |
Austria |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE13606 |
UV- or oxidized lipid treated dermal skin fibroblasts |
|
