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Sample GSM341694 Query DataSets for GSM341694
Status Public on Jan 22, 2009
Title Expression data from BAT of the KRAP deficient mice (WT1)
Sample type RNA
 
Source name brown adipose tissue
Organism Mus musculus
Characteristics C57BL6J-backcrossed, male
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA followed by purification using Rneasy kit was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Description WT1-BAT
Data processing The data were analyzed with GeneChip Operating Software (GCOS) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Nov 13, 2008
Last update date Aug 28, 2018
Contact name takahiro fujimoto
E-mail(s) [email protected]
Organization name fukuoka university
Department Faculty of medicine,Department of cell biology
Street address 7-45-1 nanakuma,jonan-ku
City Fukuoka
State/province Fukuoka
ZIP/Postal code 814-0180
Country Japan
 
Platform ID GPL1261
Series (2)
GSE13582 Expression data from BAT of the KRAP deficient mice
GSE13585 Expression data from BAT and liver of the KRAP deficient mice
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE For each transcript represented on the probe array, the algorithm computes a Signal.
ABS_CALL For each transcript represented on the probe array, the algorithm computes a Detection call.
DETECTION P-VALUE For each transcript represented on the probe array, the algorithm computes a Detection p-value.

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 131.36 P 0.000581214
AFFX-BioB-M_at 181.809 P 4.42873e-05
AFFX-BioB-3_at 113.506 P 4.42873e-05
AFFX-BioC-5_at 392.048 P 7.00668e-05
AFFX-BioC-3_at 433.136 P 4.42873e-05
AFFX-BioDn-5_at 1102.64 P 4.42873e-05
AFFX-BioDn-3_at 2498.24 P 6.02111e-05
AFFX-CreX-5_at 5609.42 P 5.16732e-05
AFFX-CreX-3_at 6393.59 P 4.42873e-05
AFFX-DapX-5_at 141.126 P 7.00668e-05
AFFX-DapX-M_at 303.71 P 0.000389797
AFFX-DapX-3_at 412.807 P 4.42873e-05
AFFX-LysX-5_at 19.5367 P 0.00159257
AFFX-LysX-M_at 46.263 P 0.0113844
AFFX-LysX-3_at 95.854 P 6.02111e-05
AFFX-PheX-5_at 25.2447 P 0.000509415
AFFX-PheX-M_at 38.4738 P 0.000856509
AFFX-PheX-3_at 58.7708 P 0.000296708
AFFX-ThrX-5_at 42.7157 P 0.000662269
AFFX-ThrX-M_at 58.3376 P 0.000258067

Total number of rows: 45101

Table truncated, full table size 1375 Kbytes.




Supplementary file Size Download File type/resource
GSM341694.CEL.gz 3.4 Mb (ftp)(http) CEL
GSM341694.CHP.gz 250.3 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file
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