|
Status |
Public on Mar 31, 2019 |
Title |
SLE PBMC 2 (P6376_CD14_1) |
Sample type |
SRA |
|
|
Source name |
SLE PBMC
|
Organism |
Homo sapiens |
Characteristics |
disease state: SLE cell type: PBMC cell subset: CD14 monocytes
|
Extracted molecule |
total RNA |
Extraction protocol |
Cells were resuspended in TCL buffer, supplemented with 1 mg/mL proteinase K, and processed through the Smart-seq2 protocol (on a microfluidic device) Libraries were constructed using the Nextera XT (Illumina) library preparation kit
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
NextSeq 550 |
|
|
Description |
Processed data file: SLE.matrix
|
Data processing |
Bcls were demultiplexed using Illumina bcl2fastq (v2.20.0.422) Fastqs were aligned to hg19 using STAR Gene expression matrices were generated using Bowtie2 Genome_build: hg19 Supplementary_files_format_and_content: Matrices are provided as samples (columns) vs. genes (rows)
|
|
|
Submission date |
Sep 25, 2018 |
Last update date |
May 15, 2019 |
Contact name |
Paul Blainey |
E-mail(s) |
[email protected]
|
Organization name |
Broad Institute
|
Street address |
415 Main St
|
City |
Cambridge |
State/province |
Massachusetts |
ZIP/Postal code |
02142 |
Country |
USA |
|
|
Platform ID |
GPL21697 |
Series (1) |
GSE120442 |
Multiplexed enrichment and genomic profiling of peripheral blood cells reveal subset-specific immune signatures |
|
Relations |
BioSample |
SAMN10127691 |
SRA |
SRX4737487 |