|
| Status |
Public on Sep 05, 2018 |
| Title |
SAL 16 RS |
| Sample type |
RNA |
| |
|
| Source name |
round spermatids from control rat
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague-Dawley
rat id: rat #16
Sex: male
cell type: round spermatids
treatment: control (saline)
|
| Treatment protocol |
Male rats were gavaged with 6 mg/Kg cyclophosphamide or saline 6 days/week for 4 weeks
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Germ cell isolation protocol: germ cells were isolated by unit gravity sedimentation using the STA-PUT method and frozen at -80°C
Total RNA was extracted using TRIzol reagent and cleaned up using RNeasy mini kit columns
|
| Label |
Cy3
|
| Label protocol |
Cyanine-3 (Cy3) labelled RNA was prepared from 100ng total RNA using the miRNA complete labeling and hyb Kit (Agilent Technologies) following manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Cy3 labelled miRNA was hybridized to Agilent Sure Print Rat miRNA 8x15K Release 16.0 microarrays at 55°C for 20 hours using the miRNA complete labeling and hyb kit (Agilent Technologies) following the manufacturer's instructions.
|
| Scan protocol |
Slides were scanned with Agilent SureScan G2600D DNA microarray scanner
|
| Description |
604_S001_miRNA_1105_Oct12_1_2
round spermatids from control rat #16
|
| Data processing |
Probe intensities were processed using Feature Extraction ver. 11.5.1.1 (Agilent Technologies) software with protocol miRNA_1105_Oct12 and grid 031189_D_F_20121224.
Quantile Normalized, Log2 transformed signal intensities, baseline transformation to 1 using Genespring software ver. 14.9 (Agilent Technonogies)
|
| |
|
| Submission date |
Sep 04, 2018 |
| Last update date |
Sep 05, 2018 |
| Contact name |
Anne Marie Lucie Downey |
| Organization name |
McGill University
|
| Department |
Pharmacology and Therapeutics
|
| Lab |
Bernard Robaire
|
| Street address |
3655 Promenade Sir William Osler, Room 115
|
| City |
Montreal |
| State/province |
Quebec |
| ZIP/Postal code |
H3G 1Y6 |
| Country |
Canada |
| |
|
| Platform ID |
GPL14860 |
| Series (1) |
| GSE119473 |
MicroRNA profiling in male rat germ cells after chronic cyclophosphamide treatment |
|
