Two biopsies were taken from each patient; one 6mm punch biopsy was obtained from lesional skin of each patient (involved sample) and the other from non-lesional skin (uninvolved sample), taken at least 10 cm away from any active plaque. One biopsy was obtained from each healty control. The non-lesional and control biopsies were taken from the buttocks or upper thighs. Biopsies were snap-frozen in liquid nitrogen, stored at -80°C. At processing the biopsy samples were homogenized while still frozen and total RNA isolation was performed using RNeasy kit protocol (Qiagen, Chatsworth, Ca, USA).
Label
biotin
Label protocol
Procedure used standard Affymetrix protocol.
Hybridization protocol
Procedure used standard Affymetrix protocol.
Scan protocol
Procedure used standard Affymetrix protocol.
Description
511.PP
Data processing
The raw data from 180 microarrays were processed using the Robust Multichip Average (RMA) method. The expression values in the table were after adjustment of RMA expression values (on the log scale) to account for batch and sex effects.
