|
| Status |
Public on Jan 25, 2009 |
| Title |
Individual_4981_NN_sample |
| Sample type |
RNA |
| |
|
| Source name |
skin_tissue
|
| Organism |
Homo sapiens |
| Characteristics |
normal skin from controls
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Two biopsies were taken from each patient; one 6mm punch biopsy was obtained from lesional skin of each patient (involved sample) and the other from non-lesional skin (uninvolved sample), taken at least 10 cm away from any active plaque. One biopsy was obtained from each healty control. The non-lesional and control biopsies were taken from the buttocks or upper thighs.
Biopsies were snap-frozen in liquid nitrogen, stored at -80°C. At processing the biopsy samples were homogenized while still frozen and total RNA isolation was performed using RNeasy kit protocol (Qiagen, Chatsworth, Ca, USA).
|
| Label |
biotin
|
| Label protocol |
Procedure used standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Procedure used standard Affymetrix protocol.
|
| Scan protocol |
Procedure used standard Affymetrix protocol.
|
| Description |
4981.NN
|
| Data processing |
The raw data from 180 microarrays were processed using the Robust Multichip Average (RMA) method. The expression values in the table were after adjustment of RMA expression values (on the log scale) to account for batch and sex effects.
|
| |
|
| Submission date |
Oct 27, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Goncalo Abecasis |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Michigan
|
| Department |
Biostatistics
|
| Street address |
1420 Washington Heights
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48105 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE13355 |
Gene expression data of skin from psoriatic patients and normal controls |
|
| Relations |
| Reanalyzed by |
GSM973079 |
| Reanalyzed by |
GSE119087 |
