|
| Status |
Public on Oct 27, 2004 |
| Title |
Corneal Keratocyte-1 |
| Sample type |
RNA |
| |
|
| Source name |
cultured corneal stromal cells
|
| Organism |
Mus musculus |
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA of the primary cultured keratocytes was extracted using TRIzol reagent.
|
| Label protocol |
Targets were produced using standard Affymetrix procedures from 5ug of total RNA.
|
| |
|
| Hybridization protocol |
using standard Affymetrix procedures
|
| Scan protocol |
using standard Affymetrix procedures
|
| Description |
Corneal stromal cells were isolated from 7-8 weeks old CD-1 mouse and cultured in DMEM containing 1% platelet-poor horse serum as described in our paper. Total RNA of the primary cultured keratocytes was extracted using TRIzol reagent. Targets were produced using standard Affymetrix procedures from 5ug of total RNA. The expression values of all probe sets are scaled to 150 of Target Intensity by using Affymetrix Microarray Suite 5.0. Three replicates of corneal keratocytes were analyzed.
Keywords = corneal stroma fibroblast
|
| Data processing |
The model-fitting DNA chip-analyzer software (dChip; Affymetrix Inc.) and an invariant-set normalization method were used for data normalization and to calculate a model-based expression value for each transcript.
The data table below reports both the MAS5.0- and dChip-calculated data.
|
| |
|
| Submission date |
Oct 22, 2004 |
| Last update date |
Jul 25, 2008 |
| Contact name |
Shukti Chakravarti |
| E-mail(s) |
[email protected]
|
| Phone |
410-502-7627
|
| Fax |
410-614-4834
|
| Organization name |
Johns Hopkins University School of Medicine
|
| Department |
Medicine
|
| Lab |
935 Ross Bldg/ GI division
|
| Street address |
720 Rutland Ave
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
| |
|
| Platform ID |
GPL81 |
| Series (1) |
| GSE1867 |
mouse corneal stromal cells |
|
