|
| Status |
Public on Oct 16, 2008 |
| Title |
FEMX-I control melanoma cells (II) |
| Sample type |
RNA |
| |
|
| Source name |
lymph node melanoma metastasis
|
| Organism |
Homo sapiens |
| Characteristics |
Originating from a lymph node metastasis in a patient, uniquely and selectively produced extrapulmonary metastases after i.v. injection of cells prepared from xenografts into adult, nude mice.
|
| Biomaterial provider |
Aurelio Lorico
|
| Growth protocol |
Cultured in RPMI (Mediatech Inc. Herndon, VA) supplemented with 10% FBS (Atlanta Biologicals, Lawrenceville, GA) at 37 °C in a 5% CO2 humidified incubator.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol phenol-based extraction.
|
| Label |
biotin
|
| Label protocol |
Biotin incorporated during in vitro transcription.
|
| |
|
| Hybridization protocol |
Biotinylated UTP complementary RNA (cRNA) probes were prepared, fragmented and hybridized to the microarrays for 16-18 h with constant rotation.
|
| Scan protocol |
The microarray slides were washed under stringent conditions, stained with Streptavidin-Alexa-647 (Invitrogen), and scanned using an Axon GenePix 4000B scanner.
|
| Description |
FEMX-I control melanoma cells (II)
|
| Data processing |
For data analyses, the local background was subtracted and the spot intensities were log2-transformed. The spot intensities were then normalized by subtracting the 70th percentile of spot intensity of the probes against human constitutive exons and adding back a scaling factor (grand mean of 70th percentiles).
|
| |
|
| Submission date |
Oct 09, 2008 |
| Last update date |
Oct 15, 2008 |
| Contact name |
Keith S Hoek |
| E-mail(s) |
[email protected]
|
| URL |
http://www.jurmo.ch/work.html
|
| Organization name |
Currently unaffilliated
|
| Street address |
private
|
| City |
Zürich |
| ZIP/Postal code |
8004 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL7430 |
| Series (1) |
| GSE13144 |
Knockdown of CD133 in melanoma cells |
|
