|
Status |
Public on Oct 08, 2008 |
Title |
control retina T0-sample7 |
Sample type |
RNA |
|
|
Source name |
unlesioned control retina
|
Organism |
Danio rerio |
Characteristics |
wild type, adult Danio retina
|
Extracted molecule |
total RNA |
Extraction protocol |
Pico Pure isolation kit (Arcturus, Molecular Devices Corporation)
|
Label |
biotin
|
Label protocol |
mRNA was amplified from total RNA using the RiboAmp HS RNA amplification kit (Arcturus, Molecular Devices Corporation.
|
|
|
Hybridization protocol |
Following fragmentation, 5ug of cRNA were hybridized on GeneChip Zebrafish Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
Description |
Gene Expression from unlesioned control retina.
|
Data processing |
The data were analyzed with BRB Array Tools 3.6 using default analysis settings and global scaling for RMA normalization.
|
|
|
Submission date |
Oct 06, 2008 |
Last update date |
Oct 07, 2008 |
Contact name |
Peter F. Hitchcock |
E-mail(s) |
[email protected]
|
Phone |
734-763-8169
|
Fax |
734-936-7231
|
Organization name |
University of Michigan
|
Department |
Ophthy and Vis Sci
|
Street address |
1000 Wall Street
|
City |
Ann Arbor |
State/province |
MI |
ZIP/Postal code |
48104 |
Country |
USA |
|
|
Platform ID |
GPL1319 |
Series (1) |
GSE13068 |
Identification of the molecular signatures integral to regenerating photoreceptors in the retina of the zebrafish |
|