|
| Status |
Public on Sep 03, 2019 |
| Title |
DEL3 Hx |
| Sample type |
RNA |
| |
|
| Source name |
DEL3 Hx
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Lung
cell line: A549
rin number: 7.8
genotype/variation: NLUCAT1 CRISPR/Cas9-deleted
culture conditions: hypoxia
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extracted using TRIzol reagent (Invitrogen)
|
| Label |
Cy3
|
| Label protocol |
Agilent - Low RNA input QuickAmp
|
| |
|
| Hybridization protocol |
Agilent : 200.0 ng at 65 degree_C during 17 hours
|
| Scan protocol |
Arrays were scanned using the Agilent SureScan High Resolution Scanner.
|
| Description |
RNA extracted from NLUCAT1 CRISPR/Cas9-deleted A549 clones cultured in hypoxia (replicate 3)
|
| Data processing |
Feature extraction data processing. Quantile normalization and differential analysis using Limma package in R / Bioconductor.
|
| |
|
| Submission date |
Jul 12, 2018 |
| Last update date |
Sep 03, 2019 |
| Contact name |
Kevin Lebrigand |
| Organization name |
IPMC/CNRS
|
| Lab |
Functional Genomics Platform of Nice-Sophia-Antipolis, France.
|
| Street address |
660 route des lucioles
|
| City |
Valbonne - Sophia-Antipolis |
| ZIP/Postal code |
06560 |
| Country |
France |
| |
|
| Platform ID |
GPL17077 |
| Series (2) |
| GSE117036 |
Impact of NLUCAT1 deletion on A549 cells in normoxic and hypoxic conditions |
| GSE117049 |
The nuclear hypoxia-regulated NLUCAT1 long non-coding RNA contributes to an aggressive phenotype in lung adenocarcinoma through regulation of oxidative stress |
|
