|
| Status |
Public on Dec 03, 2019 |
| Title |
M_15659 BALB\c NeuT+ 12 weeks |
| Sample type |
RNA |
| |
|
| Source name |
Plasma
|
| Organism |
Mus musculus |
| Characteristics |
gender: female
genotype: BALB\c NeuT+
age (weeks): 12
tumor grade: severe dys
tumor burden: 192071
hemolyzed: y
abs_414nm: 0.211
batch: 1
|
| Treatment protocol |
MMTV-NeuT transgenic females, and age-matched non transgenic siblings, were sacrified at 6, 12 and 24 weeks of age.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from plasma samples using the commercial column-based system Qiagen miRNeasy® Mini Kit (Qiagen, Valencia, CA, USA).
|
| Label |
Cy3
|
| Label protocol |
Total RNA from each sample was labeled according to the manufacturers recommendations.
|
| |
|
| Hybridization protocol |
To assess the expression levels of microRNAs Illumina mouseWG-6 v2.0 microarrays designed on miRBase 19.0 from Agilent Technologies were used.
|
| Scan protocol |
Microarrays from Agilent were scanned with an Agilent SureScan scanner, and raw data were collected using Agilent’s Feature Extraction software v10.7.
|
| Data processing |
Raw data were preprocessed using an optimized version of the MA algorithm implemented in the AgiMicroRna package (Lopez-Romero 2011). For circulating microRNAs data were log2-transformed only, as standard normalization strategies are not suitable for circulating microRNA data.
|
| |
|
| Submission date |
Jul 12, 2018 |
| Last update date |
Dec 05, 2019 |
| Contact name |
Matteo Milani |
| Organization name |
IRCCS Istituto Nazionale dei Tumori
|
| Street address |
Via Amadeo, 42
|
| City |
Milan |
| State/province |
Milan |
| ZIP/Postal code |
20133 |
| Country |
Italy |
| |
|
| Platform ID |
GPL17912 |
| Series (2) |
| GSE117000 |
Circulating miRNA profile of MMTV-NeuT mouse model of spontaneous mammary carcinogenesis. |
| GSE117071 |
Gene expression profile of bone marrow and circulating miRNA profile from MMTV-NeuT mouse model of spontaneous mammary carcinogenesis. |
|
