|
| Status |
Public on Jul 03, 2018 |
| Title |
GBMX16_2 |
| Sample type |
RNA |
| |
|
| Source name |
GBMX16
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: GBMX16 patient-derived xenoline
genotype/variation: scrambled shRNA (control)
|
| Treatment protocol |
pLKO.1 plasmids containing gene-specific or scrambled shRNA (control) were used to knockdown (KD) BRG1 in the GBMX16 patient-derived xenoline.
|
| Growth protocol |
GBMX16 patient-derived xenoline was maintained as xenografts in immunocompromised mice. Glioma-Initiating Cells were isolated and maintained in flasks precoated with poly-D-lysine and laminin, and grown in NeuroBasal-A medium (Invitrogen, Carlsbad, CA) containing 2% B27 supplement, 2 mM L-glutamine, 100 units/ml penicillin, 100 g/ml streptomycin, EGF (20 ng/ml), and basic FGF (40 ng/ml).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from GBMX16 and the GBMX16 BRG1 KD cells was extracted using the QIAshredder and RNeasy mini kits
|
| Label |
n/a
|
| Label protocol |
n/a
|
| |
|
| Hybridization protocol |
Nanostring arrays were conducted against the Immunology panels on the nCounter Analysis system
|
| Scan protocol |
standard NanoString protocol
|
| Data processing |
data was analyzed with nSolver software using a 2-fold cutoff value; mRNA counts were normalized to the geometric means of 40 housekeeping genes
|
| |
|
| Submission date |
Jul 02, 2018 |
| Last update date |
Jul 03, 2018 |
| Contact name |
Meiyun Fan |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Tennessee
|
| Street address |
19 S. Manassas Street
|
| City |
Memphis |
| ZIP/Postal code |
38163 |
| Country |
USA |
| |
|
| Platform ID |
GPL25262 |
| Series (1) |
| GSE116545 |
Chromatin remodeling factor BRG1 regulates stemness and chemosensitivity of glioma initiating cells |
|
