|
| Status |
Public on Jun 23, 2021 |
| Title |
sh52 1 |
| Sample type |
RNA |
| |
|
| Source name |
sh52 1
|
| Organism |
Homo sapiens |
| Characteristics |
shRNA: sh52
cell line: U87MG
|
| Growth protocol |
Cells were grown to subconfluence for 5 days after drug selection was completed
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was harvested with an RNeasy Kit from Qiagen
|
| Label |
biotin
|
| Label protocol |
Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Samples were hybridized with GeneChip HTA 2.0 (Affymetrix) and scanned at the Case Western Reserve University Gene Expression Core Facility.
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
| Description |
RMA expression value derived from Expression Console software; core-exon analysis
|
| Data processing |
Transcriptome Analysis Console software from Affymetrix was used.
|
| |
|
| Submission date |
Jun 28, 2018 |
| Last update date |
Jun 23, 2021 |
| Contact name |
Scott Welford |
| E-mail(s) |
[email protected]
|
| Phone |
3052438337
|
| Organization name |
University of Miami
|
| Street address |
1550 10th Avenue, Pap 503
|
| City |
Miami |
| State/province |
FL |
| ZIP/Postal code |
33136 |
| Country |
USA |
| |
|
| Platform ID |
GPL17586 |
| Series (1) |
| GSE116395 |
Identification of SAT1 regulated genes in GBM cells by microarray |
|
