|
| Status |
Public on Aug 21, 2009 |
| Title |
Chlamydia pneumoniae infected (10) |
| Sample type |
RNA |
| |
|
| Source name |
Chlamydia pneumoniae infected
|
| Organism |
Homo sapiens |
| Characteristics |
infection: Chlamydia pneumoniae
tissue: Dendritic cells derived from human monocytes
|
| Treatment protocol |
C. pneumoniae infection
|
| Growth protocol |
Six days RPMI, 2% autologous human serum, IL-4, GM-CSF
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAeasy
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at conditions recommended by the manufacturer. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GCS 3000.
|
| Description |
Chlamydia pneumoniae infected (10)
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
|
| |
|
| Submission date |
Sep 16, 2008 |
| Last update date |
Mar 24, 2010 |
| Contact name |
Robert Geffers |
| E-mail(s) |
[email protected]
|
| Phone |
+49 531-6181-3058
|
| Organization name |
HCI - Helmholtz Centre for Infection Research
|
| Department |
Dep. Molecular Bacteriology
|
| Lab |
Genome Analytics
|
| Street address |
Inhoffenstr. 7
|
| City |
Braunschweig |
| ZIP/Postal code |
38124 |
| Country |
Germany |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE12806 |
Inhibition of Chlamydia pneumoniae Replication in Human Dendritic cells by TNF-alpha-Induced Indoleamine 2,3-Dioxygenase |
|
