|
| Status |
Public on Jun 01, 2019 |
| Title |
Test-2, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
PRSS8 OE2
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HCT116
genotype/variation: overexpressing PRSS8
|
| Treatment protocol |
HCT116 cells were transiently transfected with PRSS8 expression plasmids or the vector control and collected
|
| Growth protocol |
Human colon cancer HCT116 cells from American Type Culture Collection (ATCC) (Manassas, VA) were maintained in a complete MEM medium.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
The sample labeling, microarray hybridization and washing were performed based on the manufacturer’s standard protocols.
|
| |
|
| Hybridization protocol |
Followed fragmentation and biotin labeling
|
| Scan protocol |
After washing and staining, the arrays were scanned by the Affymetrix Scanner 3000(Affymetrix).
|
| Data processing |
Affymetrix GeneChip Command Console (version 4.0, Affymetrix) software was used to extract raw data. Next, Expression Console (version1.3.1, Affymetrix) software offered RMA normalization for both gene and exon level analysis.
|
| |
|
| Submission date |
Jun 11, 2018 |
| Last update date |
Jun 01, 2019 |
| Contact name |
Wancai Yang |
| E-mail(s) |
[email protected]
|
| Phone |
3123554154
|
| Organization name |
University of Illinois at Chicago
|
| Department |
Pathology
|
| Street address |
840 South Wood Street
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60612 |
| Country |
USA |
| |
|
| Platform ID |
GPL17586 |
| Series (1) |
| GSE115566 |
PRSS8 Suppresses Colorectal Carcinogenesis and Metastasis. |
|
