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Sample GSM316712 Query DataSets for GSM316712
Status Public on Aug 29, 2008
Title CN_AML Patient 224 (U133 Plus 2.0)
Sample type RNA
 
Source name peripheral blood mononuclear cells
Organism Homo sapiens
Characteristics AML normal karyotype (test set) FAB M1; age =69 years; OS = 624 days; status (0=alive/1=dead): 1
Treatment protocol Pretreatment bone marrow or peripheral blood mononuclear cells from patients with newly diagnosed acute myeloid leukemia (AML) with a normal karyotype
Extracted molecule total RNA
Extraction protocol Mononuclear cells from bone marrow (BM) or peripheral blood (PB) were enriched by Ficoll gradient centrifugation. Total RNA was isolated using the RNeasy Mini Kit (Qiagen, Hilden, Germany). cDNA preparation and in vitro transcription were performed according to standard Affymetrix protocols.
Label biotin
Label protocol Biotin labeling of antisense cRNA was performed according to standard Affymetrix protocols
 
Hybridization protocol Sample hybridisation was performed for 16 hours according to standard Affymetrix protocols
Scan protocol Samples were scanned using an Affymetrix GeneChip scanner
Description no additional information
Data processing Normalization was performed using the variance stabilizing normalization algorithm, and probe set expression values were calculated by the median polish method. Quality control consisted of visual inspection of the array image for artifacts, assessment of RNA degradation plots, and inspection of rank-vs.-residual plots after normalization and probe set summarization.
 
Submission date Aug 29, 2008
Last update date Nov 14, 2018
Contact name Klaus H Metzeler
E-mail(s) [email protected]
Organization name University of Munich
Department Department of Internal Medicine III
Lab Laboratory for Leukemia Diagnostics
Street address Marchioninistr. 15
City Munich
ZIP/Postal code 81377
Country Germany
 
Platform ID GPL570
Series (1)
GSE12417 Prognostic gene signature for normal karyotype AML
Relations
Reanalyzed by GSE119087
Reanalyzed by GSE122511

Data table header descriptions
ID_REF
VALUE Expression values after variance stabilizing normalization and probe set summarization are provided.

Data table
ID_REF VALUE
1007_s_at 6.411948978
1053_at 7.506610421
117_at 6.438051777
121_at 8.388840011
1255_g_at 4.927917201
1294_at 9.735300189
1316_at 6.582395635
1320_at 6.231901257
1405_i_at 10.85495269
1431_at 6.104455059
1438_at 6.299829762
1487_at 7.517146355
1494_f_at 6.297980856
1552256_a_at 7.785907714
1552257_a_at 8.044097152
1552258_at 6.742361985
1552261_at 6.383243188
1552263_at 6.526824944
1552264_a_at 8.233791809
1552266_at 6.112833844

Total number of rows: 54675

Table truncated, full table size 1211 Kbytes.




Supplementary file Size Download File type/resource
GSM316712.CEL.gz 4.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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