|
| Status |
Public on Dec 22, 2008 |
| Title |
Log-phase mutant bacteria grown at 30C |
| Sample type |
RNA |
| |
|
| Source name |
Mutant bacteria of log-phase cultures grown at 30C
|
| Organism |
Shigella flexneri |
| Characteristics |
strain: shigella flexneri 2a str. 2457T: a virulence plasmid-free strain (delta pSF strain)
genotype: mutant
growth phase: log-phase
temperature: 30C
|
| Treatment protocol |
Mutant bacteria were cultured into log-phase (OD=0.6) at 30 degree
|
| Growth protocol |
Mutant bacteria were maintained at TSA plates at 30 degree and cultured in LB broth at 30 degree
|
| Extracted molecule |
total RNA |
| Extraction protocol |
use QIAGEN RNAeasy mini kit
|
| Label |
Cy3
|
| Label protocol |
Available at manufacturer's website: www.nimblegen.com
|
| |
|
| Hybridization protocol |
Available at manufacturer's website: www.nimblegen.com
|
| Scan protocol |
Available at manufacturer's website: www.nimblegen.com
|
| Description |
Log-phase mutant bacteria grown at 30 degree
|
| Data processing |
Available at manufacturer's website: www.nimblegen.com
|
| |
|
| Submission date |
Aug 21, 2008 |
| Last update date |
Jun 02, 2010 |
| Contact name |
Li Zhu |
| E-mail(s) |
[email protected]
|
| Organization name |
Beijing Institute of Biotechnology
|
| Department |
State Key Laboratory of Pathogens and Biosecurity
|
| Street address |
20 Dongdajie, Fengtai District
|
| City |
Beijing |
| ZIP/Postal code |
100071 |
| Country |
China |
| |
|
| Platform ID |
GPL7185 |
| Series (1) |
| GSE12535 |
The expression profiles analysis of a virulence plasmid-cured strain of shigella flexneri |
|
