|
| Status |
Public on Jul 31, 2011 |
| Title |
Subject 4; two months before the beginning of heating experiment |
| Sample type |
RNA |
| |
|
| Source name |
Skeletal muscle
|
| Organism |
Homo sapiens |
| Characteristics |
Gender: Male
Tissue: vastus lateralis muscle
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction was performed by using ISOGEN (Nippon Gene Co., LTD, Toyama, Japan) according to the manufacturer's instructions, and purified using an RNeasy Mini kit (Qiagen K.K, Tokyo, Japan).
|
| Label |
biotin
|
| Label protocol |
Preparation of labeled cRNA from the RNA was performed according to Affymetrix Two-Cycle Target Labeling Assay.
|
| |
|
| Hybridization protocol |
Following fragmentation, the 15 µg labeled cRNA hybridized for 16 hr at 45°C to Human Genome U133 Plus 2.0 Array (Affymetrix, Santa Clara, CA) according to the manufacturer’s instructions. After stringent washing in a fluidics station 450, cRNA was stained with R-phycoerythrin streptavidine and scanned after antibody amplification.
|
| Scan protocol |
Fluorescence intensities were measured with an Affymetrix Scanner.
|
| Description |
Image output files were inspected for hybridization artifacts, and based on the fluorescence intensity differences between perfect match and mismatch probes.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Aug 18, 2008 |
| Last update date |
Jul 31, 2011 |
| Contact name |
Hidehiko Kondo |
| E-mail(s) |
[email protected]
|
| Phone |
81-285-68-7769
|
| Fax |
81-285-68-7495
|
| Organization name |
Kao Corporation
|
| Department |
Biological Science Laboratories
|
| Street address |
2606 Akabane, Ichikai-Machi
|
| City |
Haga-Gun, Tochigi |
| ZIP/Postal code |
321-3497 |
| Country |
Japan |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE12474 |
Microarray analysis of skeletal muscle hypertrophy induced by heat-stress in healthy humans |
|
