|
| Status |
Public on Nov 03, 2008 |
| Title |
[OBF-1]-2 |
| Sample type |
RNA |
| |
|
| Source name |
Bone marrow
|
| Organism |
Mus musculus |
| Characteristics |
Strain: Bl6, Tissue: Bone marrow, Age: 7 weeks
|
| Treatment protocol |
Bone marrow was extracted from the mice
|
| Growth protocol |
Mice were grown for seven weeks in our mouse facility
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was purified using the Qiagen RNA easy protocol according to the manufacturer's insructions
|
| Label |
Biotin
|
| Label protocol |
RNA was amplified and labelled using the Affymetrix 1-cycle 3'IVT protocol
|
| |
|
| Hybridization protocol |
RNA was hybridised using the standard Affymetrix hybridisation protocol as per manufacturer's instructions (45°C over night)
|
| Scan protocol |
The chips were scanned using an Affymetrix genechip scanner as per manufacturer's instructions
|
| Description |
Bone marrow from an OBF-1 deletion mouse
|
| Data processing |
Expression values were estimated using the RMA algorithm implemented in Bioconductor. Further data anaylsis was performed using GeneSpring 4
|
| |
|
| Submission date |
Aug 06, 2008 |
| Last update date |
Nov 03, 2008 |
| Contact name |
Edward James Oakeley |
| E-mail(s) |
[email protected]
|
| Organization name |
Friedrich Miescher Institute for Biomedical Research
|
| Street address |
Maulbeerstrasse 66
|
| City |
Basel |
| ZIP/Postal code |
4058 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL339 |
| Series (1) |
| GSE12356 |
Analysis of Aiolos and OBF-1 deletions in bone marrow from 7 week old mice |
|
