|
| Status |
Public on Aug 06, 2008 |
| Title |
WT sham 4 |
| Sample type |
RNA |
| |
|
| Source name |
sham-operated wild-type mice
|
| Organism |
Mus musculus |
| Characteristics |
Three-month-old male PPARa-/- mice
|
| Treatment protocol |
Wild-type and PPARalpha-/- mice were sham-operated or subjected to TAC for 28 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
One-cycle target labeling according to Affymetrix manual
|
| |
|
| Hybridization protocol |
Biotin labeled target complementary RNA (cRNA) was fractionated and hybridized to Affymetrix 430 2.0 Mouse Genome GeneChip according to manufacturer’s instructions.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip scanner 3000 / 7G
|
| Description |
Gene expression data from sham-operated wild-type mice
|
| Data processing |
Affymetrix GeneChip Operating Software (GCOS, version 1.4) was used to analyze image data. For each transcript represented on the array by a probe set, the expression algorithm computed the detection call (present, absent, or marginal), the detection p-value, and the signal which is an average intensity value for each probe set.
|
| |
|
| Submission date |
Aug 05, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Marc van Bilsen |
| E-mail(s) |
[email protected]
|
| Organization name |
Universiteit Maastricht
|
| Street address |
PO BOX 616
|
| City |
Maastricht |
| ZIP/Postal code |
6200MD |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE12337 |
Transcriptomic analysis of PPARalpha-dependent alterations during cardiac hypertrophy |
|
| Relations |
| Reanalyzed by |
GSE119085 |
