|
| Status |
Public on Nov 13, 2018 |
| Title |
GSS0392_112_M_1800 |
| Sample type |
RNA |
| |
|
| Source name |
batch_subjectID_gender_sampling time
|
| Organism |
Homo sapiens |
| Characteristics |
Sex: Male
tissue: LCM-separated epidermis
ancestry: NA
collection time: 1800
|
| Treatment protocol |
Punch biopsy samples were frozen in OCT media. Laser capture microdissection (LCM) was performed to isolate the epidermal compartments for gene expression profiling.
|
| Growth protocol |
n/a
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from LCM samples was isolated utilizing the Pico Pure RNA Isolation Kit (Life Technologies, Grand Island, NY) according to manufacturer's recommendations
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared using the Affymetrix HT 3’ IVT Express kit (Cat. #901253) on a Beckman Biomek FXp Laboratory Automation Workstation (Beckman Cat. #A31842).
|
| |
|
| Hybridization protocol |
Biotinylated cRNA was fragmented by limited alkaline hydrolysis and then hybridized overnight to Affymetrix GeneTitan U219 microarray plates using the Affymetrix GeneTitan instrument
|
| Scan protocol |
ArrayPlates were scanned using the Affymetrix GeneTitan Instrument utilizing AGCC GeneTitan Instrument Control software version 4.2.0.1566
|
| Description |
Gene expression data from LCM-separated epidermis from human forearm
|
| Data processing |
The RMA algorithm was used as the processing protocol to normalize the data for subsequent analysis.
|
| |
|
| Submission date |
Apr 04, 2018 |
| Last update date |
Nov 13, 2018 |
| Contact name |
Joe Sherrill |
| E-mail(s) |
[email protected]
|
| Phone |
5136222038
|
| Organization name |
Procter and Gamble
|
| Street address |
8700 Mason-Montgomery Rd
|
| City |
Mason |
| State/province |
Ohio |
| ZIP/Postal code |
45040 |
| Country |
USA |
| |
|
| Platform ID |
GPL13667 |
| Series (1) |
| GSE112660 |
The effect of circadian rhythm on gene expression in human skin |
|
