|
| Status |
Public on Jul 25, 2008 |
| Title |
neonatal cerebellum, T9H/+ matdup proximal Chr 7, patdup proximal Chr 15, 430B |
| Sample type |
RNA |
| |
|
| Source name |
neonatal cerebellum, T9H/+ matdup proximal Chr 7, patdup proximal Chr 15
|
| Organism |
Mus musculus |
| Characteristics |
Genotype: T9H/+ heterozygotes
|
| Growth protocol |
Neonatal mice and embryos were bred and identified as described (Buettner, VL, et al., Mammalian Genome, volume 16, 219-227 (2005).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared by use of Trizol followed by the RNeasy Mini Kit as described in the manufacturers' instructions.
|
| Label |
biotin
|
| Label protocol |
Labeling, hybridization, scanning and data processing were performed at the UC Irvine DNA and Protein Microarray Facility. Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 9 µg total RNA (GeneChipR Expression Analysis Technical Manual, 2001, Affymetrix, Inc., Santa Clara, CA).
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| |
|
| Hybridization protocol |
Following fragmentation, 10 micrograms of fragmented target cRNA was hybridized for 16 hr at 45° C on GeneChipR Mouse Expression Array 430B. Genechips were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard Gen/Array Scanner G2500A.
|
| Description |
expression data from neonatal cerebellum
|
| Data processing |
Data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling with a target intensity value of 2500 as the normalization method.
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| |
|
| Submission date |
Jul 24, 2008 |
| Last update date |
Jul 24, 2008 |
| Contact name |
Xiwei Wu |
| E-mail(s) |
[email protected]
|
| Organization name |
City of Hope National Medical Center
|
| Department |
Computational and Quantitative Medicine
|
| Street address |
1500 E. Duarte Rd.
|
| City |
Duarte |
| State/province |
CA |
| ZIP/Postal code |
91010 |
| Country |
USA |
| |
|
| Platform ID |
GPL340 |
| Series (2) |
| GSE12230 |
Neonatal and embyronic CNS of mice with maternal or paternal duplication of proximal chromosomes 7 and 15 (430B) |
| GSE12231 |
Neonatal and embyronic CNS of mice with maternal or paternal duplication of proximal chromosomes 7 and 15 |
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