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Sample GSM3073219

Query DataSets for GSM3073219

Status

Public on Apr 02, 2018

Title

BAT_cold_4

Sample type

SRA

Source name

BAT_cold

Organism

Mus musculus

Characteristics

strain: C57BL/6
genotype/variation: wild type
gender: female
age: 16w
kept at: 5°C
tissue: Inguinal BAT

Treatment protocol

All mice were bred, born and weaned at 30°C. At the age of 10-12 weeks, half of the mice were transferred to a temperature-controlled (+/- 0.5°C) climate chamber and kept for two weeks at 18°C followed by 3 weeks at 5°C. Afterwards, mice kept at 30°C and 5°C were euthanized after 2-3 hours food withdrawal, and serum and tissue samples were collected.

Extracted molecule

total RNA

Extraction protocol

RNA molecules were poly(A) selected, fragmented, and reverse transcribed with the Elute, Prime, Fragment Mix (EPF, Illumina)
End repair, A-tailing, adaptor ligation, and library enrichment were performed as described in the Low Throughput protocol of the TruSeq RNA Sample Prep Guide (Illumina).

Library strategy

RNA-Seq

Library source

transcriptomic

Library selection

cDNA

Instrument model

Illumina HiSeq 2500

Description

GAR0580_1

Data processing

Sequenced reads were trimmed for adaptor sequence with trim-galore and aligned with tophat softare
Genome_build: Ensembl release 81
Supplementary_files_format_and_content: tab-delimited text files include raw read count per sample

Submission date

Apr 01, 2018

Last update date

Apr 02, 2018

Contact name

Yiming Cheng

E-mail(s)

[email protected]

Organization name

Helmholtz Munich

Street address

Ingolstädter Landstraße 1