|
| Status |
Public on Aug 30, 2008 |
| Title |
K562 cells CREB Knock-out replicate 5 |
| Sample type |
RNA |
| |
|
| Source name |
K562 cells CREB Knock-out
|
| Organism |
Homo sapiens |
| Characteristics |
K562 (Iscoves + 10% FCS) and TF-1 (RPMI + 10%FCS + rhGM-CSF. Cells were cultured at 37oC, 5% CO2 and split every 3 to 4 days. The CREB specific shRNA sequences were selected and validated based on accepted parameters established by Tuschl et al.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133+ Array Set HG-U133A array
|
| Label |
biotin
|
| Label protocol |
RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133+ Array Set HG-U133A array
|
| |
|
| Hybridization protocol |
RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133+ Array Set HG-U133A array
|
| Scan protocol |
RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Human Genome U133+ Array Set HG-U133A array
|
| Description |
none
|
| Data processing |
MAS5.0
|
| |
|
| Submission date |
Jul 09, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
matteo pellegrini |
| E-mail(s) |
[email protected]
|
| Phone |
310 825-0012
|
| Organization name |
UCLA
|
| Street address |
610 charles young drive east
|
| City |
los angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE12056 |
Expression Profile of CREB knockdown in Myeloid Leukemia Cells |
|
| Relations |
| Reanalyzed by |
GSE119087 |
