|
| Status |
Public on Jul 10, 2008 |
| Title |
kn muscle 2, biological rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
laminin alpha 2 chain deficient mouse
|
| Organism |
Mus musculus |
| Characteristics |
dy3K/dy3K, mouse model for congenital muscular dystrophy type 1 A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIzol extraction of total RNA and further purified using the RNeasy Mini Kit (Qiagen) was used according to the manufacturers’ instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Swegene Microarray Resource Centre (Lund, Sweden).
|
| |
|
| Hybridization protocol |
After fragmentation of the cRNA, 15 μg cRNA in 200 μl hybridisation cocktail was hybridised onto Affymetrix MOE430 2.0 microarray chip for 16 h at 45 °C. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000.
|
| Description |
gene expression data from 4 weeks old laminin alpah 2 chain deficient mouse hind limb skeletal muscle
|
| Data processing |
The data were analyzed with dChip 2004. After array normalization (default analysis settings) the model-based expression values were calculated using the PM-only model
|
| |
|
| Submission date |
Jul 09, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Mattias Carl Häger |
| E-mail(s) |
[email protected]
|
| Organization name |
Lunds University
|
| Department |
Expreimental Biology
|
| Lab |
Muscle Biology
|
| Street address |
BMC, B12
|
| City |
LUND |
| State/province |
M |
| ZIP/Postal code |
221 84 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE12049 |
Expression data from laminin alpha 2 chain deficient mice vs wild type |
|
| Relations |
| Reanalyzed by |
GSE119085 |
