|
| Status |
Public on Jul 18, 2008 |
| Title |
Muscle JDM (YJD-JDM-3UA-s2) |
| Sample type |
RNA |
| |
|
| Source name |
skeletal muscle, long duration
|
| Organism |
Homo sapiens |
| Characteristics |
duration of untreated disease in month:7.7
age at onset:1.6
|
| Treatment protocol |
n/a
|
| Growth protocol |
n/a
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted according to the standard Affymetrix protocol including Trizol extraction and Rneasy column cleanup
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Description |
n/a
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 800.
|
| |
|
| Submission date |
Jul 02, 2008 |
| Last update date |
Jul 02, 2008 |
| Contact name |
Yi-Wen Chen |
| E-mail(s) |
[email protected]
|
| Organization name |
Children's Research Institute
|
| Department |
Genetic Medicine Research
|
| Street address |
111 Michigan Ave NW
|
| City |
Washington |
| State/province |
DC |
| ZIP/Postal code |
20010 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE11971 |
Skeletal muscles of untreated children with juvenile dermatomyositis |
|
