|
Status |
Public on Feb 13, 2018 |
Title |
Patient Derived Xenograft LTL-418R |
Sample type |
RNA |
|
|
Source name |
Prostate Tumor
|
Organism |
Homo sapiens |
Characteristics |
tissue: prostate cancer tumor tumor foci: Patient Derived Xenograft
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA were extracted by using the mirVana RNA Isolation Kit (Ambion, Burlington, Canada). The quality and quantity of RNA were assessed with an Agilent 2100 Bioanalyzer (Caliper Technologies Corp., Canada)
|
Label |
Cy3
|
Label protocol |
100ng of total RNA was used as input for generation of Cyanine-3-labeled cRNA following Agilent’s One-Color Microarray-Based Gene Expression Analysis Low Input Quick Amp Labeling v6.0.
|
|
|
Hybridization protocol |
600ng labeled cRNA was hybridized on Agilent SurePrint G3 Human GE 8x60K Microarray (Design ID 028004)
|
Scan protocol |
Slides were scanned with the Agilent DNA Microarray Scanner at a 3um scan resolution using the one colour and 20 bit tiff file settings
|
Data processing |
Image files were processed with Agilent Feature Extraction 10.10 to produce spatially detrended and background subtracted gProcessedSignal intensities. Data were quantile normalized in GeneSpring 12.0.
|
|
|
Submission date |
Feb 13, 2018 |
Last update date |
Feb 13, 2018 |
Contact name |
Shawn Anderson |
E-mail(s) |
[email protected]
|
Organization name |
Vancouver Prostate Centre
|
Lab |
Laboratory for Advanced Genome Analysis
|
Street address |
2660 Oak Street
|
City |
Vancouver |
State/province |
BC |
ZIP/Postal code |
V6H3Z6 |
Country |
Canada |
|
|
Platform ID |
GPL14550 |
Series (2) |
GSE41192 |
High fidelity patient-derived xenografts for accelerating prostate cancer discovery and drug development (expression) |
GSE41193 |
High fidelity patient-derived xenografts for accelerating prostate cancer discovery and drug development |
|