mRNA extracted from control lung fibroblasts derived from normal periphery of resected tumor. Lung fibroblasts were grown to confluence in DMEM with 10% fetal calf serum. At confluence, lung fibroblasts were serum-deprived overnight, and exposed to 4 ng/ml of activated TGF-ß1 (R&D Systems) in serum-free culture-medium with the addition of 0.1% BSA for 4 hours. At the end of treatment period total RNA was harvested, quantified, and integrity was verified by denaturing gel electrophoresis.
